Christian Damgaard - Research Associate
I am currently working in the Lykke-Andersen lab, where the main body of research is concerned with the regulation of post transcriptional gene expression. The lab studies regulated mRNA decay including ARE-mRNA decay and non-sense mediated mRNA decay (NMD). One major focus has been put on the sub-cellular localization of various mRNA decay processes. Many factors encoded by ARE-mRNAs are usually potent cellular "effector" proteins. For example, many ARE-mRNAs encode proto-oncogenes, growth factors, cytokines and tumor suppressors. In line with this, mis-regulation of ARE-mRNA decay has been found associated with a number of pathologies including cancers and auto-immune diseases. Numerous trans factors interact with AREs to affect their turnover and translation. These are commonly called AU-rich binding proteins (AUBPs). One of my current projects, which is sponsored by the Benzon Foundation, Denmark, is to characterize a panel of AUBPs and delineate how their activity is regulated by cellular signaling. Another project aims to characterize factors important for regulating translation and stability of 5' terminal oligopyrimidine tract containing mRNAs during stress.
Paca-Uccaralertkun S, Damgaard CK, Auewarakul P, Thitithanyanont A, Suphaphiphat P, Essex M, Kjems J, Lee TH, "The Effect of a Single Nucleotide Substitution in the Splicing Silencer in the tatrev Intron on HIV Type 1 Envelope Expression" AIDS research and human retroviruses 22 (2006): 76-82
Andersen ES, Contera SA, Knudsen B, Damgaard CK, Besenbacher F, Kjems J, "Role of the trans-activation response element in dimerization of HIV-1 RNA" The Journal of biological chemistry 279 (2004): 22243-9
Zahler AM, Damgaard CK, Kjems J, Caputi M, "SC35 and heterogeneous nuclear ribonucleoprotein AB proteins bind to a juxtaposed exonic splicing enhancerexonic splicing silencer element to regulate HIV-1 tat exon 2 splicing" The Journal of biological chemistry 279 (2004): 10077-84
Damgaard CK, Andersen ES, Knudsen B, Gorodkin J, Kjems J, "RNA interactions in the 5 region of the HIV-1 genome" Journal of molecular biology 336 (2004): 369-79
Jakobsen MR, Damgaard CK, Andersen ES, Podhajska A, Kjems J, "A genomic selection strategy to identify accessible and dimerization blocking targets in the 5-UTR of HIV-1 RNA" Nucleic acids research 32 (2004): e67
Andersen ES, Jeeninga RE, Damgaard CK, Berkhout B, Kjems J, "Dimerization and template switching in the 5 untranslated region between various subtypes of human immunodeficiency virus type 1" Journal of virology 77 (2003): 3020-30
Damgaard CK, Tange TO, Kjems J, "hnRNP A1 controls HIV-1 mRNA splicing through cooperative binding to intron and exon splicing silencers in the context of a conserved secondary structure" RNA (New York, N.Y.) 8 (2002): 1401-15
Poulsen H, Nilsson J, Damgaard CK, Egebjerg J, Kjems J, "CRM1 mediates the export of ADAR1 through a nuclear export signal within the Z-DNA binding domain" Molecular and cellular biology 21 (2001): 7862-71
Tange TO, Damgaard CK, Guth S, Valcárcel J, Kjems J, "The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element" The EMBO journal 20 (2001): 5748-58
Lyngsø C, Bouteiller G, Damgaard CK, Ryom D, Sanchez-Muñoz S, Nørby PL, Bonven BJ, Jørgensen P, "Interaction between the transcription factor SPBP and the positive cofactor RNF4 An interplay between protein binding zinc fingers" The Journal of biological chemistry 275 (2000): 26144-9
Damgaard CK, Dyhr-Mikkelsen H, Kjems J, "Mapping the RNA binding sites for human immunodeficiency virus type-1 gag and NC proteins within the complete HIV-1 and -2 untranslated leader regions" Nucleic acids research 26 (1998): 3667-76
