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Coronavirus derived expression systems
Coronavirus derived expression systems
5387
Both helper dependent expression systems, based on two components, and single genomes constructed by targeted recombination, or by using infectious cDNA clones, have been developed. The sequences that regulate transcription have been characterized mainly using helper dependent expression systems and it will now be possible to validate them using single genomes. The genome of coronaviruses has been engineered by modification of the infectious cDNA leading to an efficient (>20 microg ml(-1)) and stable (>20 passages) expression of the foreign gene. The possibility of engineering the tissue and species tropism to target expression to different organs and animal species, including humans, increases the potential of coronaviruses as vectors. Thus, coronaviruses are promising virus vectors for vaccine development and, possibly, for gene therapy.
Enjuanes L, Sola I, Almazan F, Ortego J, Izeta A, Gonzalez JM, Alonso S, Sanchez JM, Escors D, Calvo E, Riquelme C, Sanchez C
Journal of biotechnology
2001-07-12 00:00
88
3
183-204
Coronavirus,Gene Expression Regulation, Viral,Gene Therapy,Genetic Vectors,Genome, Viral,Humans,Transcription, Genetic,Tropism,Vaccines,Vaccines
Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma, Cantoblanco, 28049, Madrid, Spain. l.enjuanes@cnb.uam.es
J. Biotechnol.
0168-1656
S0168165601002814
0
False
11434966
