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Regulation of cardiac stress signaling by protein kinase d1
Regulation of cardiac stress signaling by protein kinase d1
3716
In response to pathological stresses such as hypertension or myocardial infarction, the heart undergoes a remodeling process that is associated with myocyte hypertrophy, myocyte death, and fibrosis. Histone deacetylase 5 (HDAC5) is a transcriptional repressor of cardiac remodeling that is subject to phosphorylation-dependent neutralization in response to stress signaling. Recent studies have suggested a role for protein kinase C (PKC) and its downstream effector, protein kinase D1 (PKD1), in the control of HDAC5 phosphorylation. While PKCs are well-documented regulators of cardiac signaling, the function of PKD1 in heart muscle remains unclear. Here, we demonstrate that PKD1 catalytic activity is stimulated in cardiac myocytes by diverse hypertrophic agonists that signal through G protein-coupled receptors (GPCRs) and Rho GTPases. PKD1 activation in cardiomyocytes occurs through PKC-dependent and -independent mechanisms. In vivo, cardiac PKD1 is activated in multiple rodent models of pathological cardiac remodeling. PKD1 activation correlates with phosphorylation-dependent nuclear export of HDAC5, and reduction of endogenous PKD1 expression with small interfering RNA suppresses HDAC5 shuttling and associated cardiomyocyte growth. Conversely, ectopic overexpression of constitutively active PKD1 in mouse heart leads to dilated cardiomyopathy. These findings support a role for PKD1 in the control of pathological remodeling of the heart via its ability to phosphorylate and neutralize HDAC5.
Harrison BC, Kim MS, van Rooij E, Plato CF, Papst PJ, Vega RB, McAnally JA, Richardson JA, Bassel-Duby R, Olson EN, McKinsey TA
Molecular and cellular biology
2006-05-01 00:00
26
10
3875-88
Animals,Animals, Newborn,COS Cells,Cardiomyopathy, Hypertrophic,Catalytic Domain,Cells, Cultured,Cercopithecus aethiops,Enzyme Activation,Gene Expression Regulation,Heart Ventricles,Isoenzymes,Male,Mice,Mice, Transgenic,Models, Biological,Myocytes, Cardiac,Protein Kinases,RNA, Small Interfering,Rats,Rats, Inbred SHR,Rats, Inbred WF,Rats, Sprague-Dawley,Signal Transduction,Stress,Isoenzymes,RNA, Small Interfering,Protein Kinases,protein kinase D1, rat
Myogen, Inc., 7575 West 103rd Ave., Westminster, Colorado 80021, USA.
Mol. Cell. Biol.
0270-7306
10.1128/MCB.26.10.3875-3888.2006
26/10/3875
0
False
16648482
