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Chromosome segregation in fission yeast ... the tubulin folding cofactor D
Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D
5343
Faithful chromosome segregation requires the combined activities of the microtubule-based mitotic spindle and the multiple proteins that form mitotic kinetochores. Here, we show that the fission yeast mitotic mutant, tsm1-512, is an allele of the tubulin folding chaperone, cofactor D. Chromosome segregation in this and in an additional cofactor D mutant depends on growth conditions that are monitored specifically by the mitotic checkpoint proteins Mad1, 2, 3 and Bub3. The temperature-sensitive mutants we have used disrupt the function of cofactor D to different extents, but both strains form a mitotic spindle in which the poles separate in anaphase. However, chromosome segregation is often unequal, apparently due to a defect in kinetochore-microtubule interactions. Mutations in cofactor D render cells particularly sensitive to the expression levels of a CENP-B-like protein, Abp1p, which works as an allele-specific, high-copy suppressor of cofactor D. This and other genetic interactions between cofactor D mutants and specific kinetochore and spindle components suggest their critical role in establishing the normal kinetochore-microtubule interface.
Fedyanina OS, Mardanov PV, Tokareva EM, McIntosh JR, Grishchuk EL
Current genetics
2006-11-01 00:00
50
5
281-94
National Research Center for Hematology, Moscow, 125167, Russia.
Curr. Genet.
0172-8083
10.1007/s00294-006-0095-9
0
False
17004072
