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Integrated genomic and proteomic analyses of gene expression in Mammalian cells
Integrated genomic and proteomic analyses of gene expression in Mammalian cells
5441
Using DNA microarrays together with quantitative proteomic techniques (ICAT reagents, two-dimensional DIGE, and MS), we evaluated the correlation of mRNA and protein levels in two hematopoietic cell lines representing distinct stages of myeloid differentiation, as well as in the livers of mice treated for different periods of time with three different peroxisome proliferative activated receptor agonists. We observe that the differential expression of mRNA (up or down) can capture at most 40% of the variation of protein expression. Although the overall pattern of protein expression is similar to that of mRNA expression, the incongruent expression between mRNAs and proteins emphasize the importance of posttranscriptional regulatory mechanisms in cellular development or perturbation that can be unveiled only through integrated analyses of both proteins and mRNAs.
Tian Q, Stepaniants SB, Mao M, Weng L, Feetham MC, Doyle MJ, Yi EC, Dai H, Thorsson V, Eng J, Goodlett D, Berger JP, Gunter B, Linseley PS, Stoughton RB, Aebersold R, Collins SJ, Hanlon WA, Hood LE
Molecular & cellular proteomics : MCP
2004-10-01 00:00
3
10
960-9
Animals,Cell Line,Culture Media, Conditioned,Electrophoresis, Gel, Two-Dimensional,Gene Expression Regulation,Genomics,Hematopoietic Stem Cells,Kinetics,Liver,Male,Mass Spectrometry,Mice,Mice, Inbred C57BL,Myeloid Progenitor Cells,Myelopoiesis,Oligonucleotide Array Sequence Analysis,Peroxisome Proliferator-Activated Receptors,Peroxisome Proliferators,Protein Processing, Post-Translational,Proteins,Proteomics,Pyrimidines,RNA, Messenger,Thiazolidinediones,Variation (Genetics),Culture Media, Conditioned,Peroxisome Proliferator-Activated Receptors,Peroxisome Proliferators,Proteins,Pyrimidines,RNA, Messenger,Thiazolidinediones,rosiglitazone,2,4-thiazolidinedione,pirinixic acid
The Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA. qtian@systemsbiology.org
Mol. Cell Proteomics
NHLBI HL 54881, NHLBI N01 HV 28179, NIDDK P01 DK 53074, NCI R33 CA 093302
1535-9476
10.1074/mcp.M400055-MCP200
M400055-MCP200
0
False
15238602
