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Differential gene expression profiling of adult murine hematopoietic stem cells
Differential gene expression profiling of adult murine hematopoietic stem cells
5441
Hematopoietic stem cells (HSCs) have self-renewal capacity and multilineage developmental potentials. The molecular mechanisms that control the self-renewal of HSCs are still largely unknown. Here, a systematic approach using bioinformatics and array hybridization techniques to analyze gene expression profiles in HSCs is described. To enrich mRNAs predominantly expressed in uncommitted cell lineages, 54 000 cDNA clones generated from a highly enriched population of HSCs and a mixed population of stem and early multipotent progenitor (MPP) cells were arrayed on nylon membranes (macroarray or high-density array), and subtracted with cDNA probes derived from mature lineage cells including spleen, thymus, and bone marrow. Five thousand cDNA clones with very low hybridization signals were selected for sequencing and further analysis using microarrays on glass slides. Two populations of cells, HSCs and MPP cells, were compared for differential gene expression using microarray analysis. HSCs have the ability to self-renew, while MPP cells have lost the capacity for self-renewal. A large number of genes that were differentially expressed by enriched populations of HSCs and MPP cells were identified. These included transcription factors, signaling molecules, and previously unknown genes.
Park IK, He Y, Lin F, Laerum OD, Tian Q, Bumgarner R, Klug CA, Li K, Kuhr C, Doyle MJ, Xie T, Schummer M, Sun Y, Goldsmith A, Clarke MF, Weissman IL, Hood L, Li L
Blood
2002-01-15 00:00
99
2
488-98
Animals,DNA, Complementary,Gene Expression Profiling,Gene Library,Hematopoiesis,Hematopoietic Stem Cells,Mice,Mice, Inbred C57BL,Oligonucleotide Array Sequence Analysis,Protein Biosynthesis,Proteins,RNA, Messenger,Subtraction Technique,DNA, Complementary,Proteins,RNA, Messenger
University of Michigan, Department of Internal Medicine, Ann Arbor, USA.
Blood
NIDDK 1P01 DK53074-02
0006-4971
0
False
11781229
