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A multiresolution approach to orientatio ... microscopy of single particles
A multiresolution approach to orientation assignment in 3D electron microscopy of single particles
7335
Three-dimensional (3D) electron microscopy (3DEM) aims at the determination of the spatial distribution of the Coulomb potential of macromolecular complexes. The 3D reconstruction of a macromolecule using single-particle techniques involves thousands of 2D projections. One of the key parameters required to perform such a 3D reconstruction is the orientation of each projection image as well as its in-plane orientation. This information is unknown experimentally and must be determined using image-processing techniques. We propose the use of wavelets to match the experimental projections with those obtained from a reference 3D model. The wavelet decomposition of the projection images provides a framework for a multiscale matching algorithm in which speed and robustness to noise are gained. Furthermore, this multiresolution approach is combined with a novel orientation selection strategy. Results obtained from computer simulations as well as experimental data encourage the use of this approach.
Sorzano CO, Jonić S, El-Bez C, Carazo JM, De Carlo S, Thévenaz P, Unser M
Journal of structural biology
2004-06-01 00:00
146
3
381-92
Algorithms,Bacteriorhodopsins,Computer Simulation,GroEL Protein,Imaging, Three-Dimensional,Macromolecular Substances,Microscopy, Electron,Models, Molecular,Particle Size,GroEL Protein,Macromolecular Substances,Bacteriorhodopsins
Escuela Politécnica Superior, Universidad San Pablo-CEU, Campus Urb., Madrid, Spain. coss@cnb.uam.es
J. Struct. Biol.
NHLBI HL70472
1047-8477
10.1016/j.jsb.2004.01.006
S1047847704000073
0
False
15099579
