Document Actions
Identification of Bordetella pertussis v ... ociated outer membrane proteins
Identification of Bordetella pertussis virulence-associated outer membrane proteins
5378
Bordetella pertussis virulence-associated 30-, 32-, 90- and 95-kDa outer membrane proteins were purified and their N-terminal amino acid sequences were determined. The 30- and 32-kDa outer membrane proteins showed identity to the C-terminal region of the precursors of the serum resistance protein (BrkA) and the tracheal colonization factor, respectively. We confirmed the cleavage site of these precursors after N731 for BrkA and after N393 for tracheal colonization factor. Associated with the 32-kDa outer membrane protein, we found a new group of 36-kDa virulence-associated peptides. The 95-kDa outer membrane protein showed identity to Vag8. The 90-kDa outer membrane protein did not show homology with the described proteins. We report the N-termini sequence of Vir-90, a novel potential virulence factor.
Passerini de Rossi BN, Friedman LE, González Flecha FL, Castello PR, Franco MA, Rossi JP
FEMS microbiology letters
1999-03-01 00:00
172
1
9-13
Amino Acid Sequence,Bacterial Outer Membrane Proteins,Bordetella pertussis,Electrophoresis, Polyacrylamide Gel,Humans,Molecular Sequence Data,Protein Precursors,Sequence Alignment,Virulence,Bacterial Outer Membrane Proteins,BrkA protein, Bordetella pertussis,Protein Precursors
Departamento de Química Biológica-IQUIFIB, Universidad de Buenos Aires, Argentina.
FEMS Microbiol. Lett.
0378-1097
S0378-1097(99)00009-9
0
False
10079522
