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cDNA cloning of human hnRNP protein A1 r ... tence of multiple mRNA isoforms
cDNA cloning of human hnRNP protein A1 reveals the existence of multiple mRNA isoforms
5161
Protein A1 is one of the major component of mammalian ribonucleoprotein particles (hnRNP). Human protein A1 cDNA cloning and sequencing revealed the existence of at least two protein isoforms. Among the cDNAs examined, sequence differences were found both in the structural portion, leading to aminoacid changes (Tyr to Phe or Arg to Lys) and in the non translated 3'-region where two T-stretches of different length were observed. Interestingly one of the aminoacid substitutions falls into a consensus sequence common to many RNA binding proteins. Northern blot analysis of poly A+ RNAs from five human tissues revealed two mRNA forms of 1500 and 1900 n due to alternative polyadenylation. Analysis of genomic DNA showed at least 30 A1-specific sequences, some of which correspond to processed pseudogenes. These results suggest that protein A1 is encoded by a multigene family.
Buvoli M, Biamonti G, Tsoulfas P, Bassi MT, Ghetti A, Riva S, Morandi C
Nucleic acids research
1988-05-11 00:00
16
9
3751-70
Amino Acid Sequence,Base Sequence,Cloning, Molecular,DNA,DNA Restriction Enzymes,Genes,Heterogeneous-Nuclear Ribonucleoprotein Group A-B,Heterogeneous-Nuclear Ribonucleoproteins,Humans,Molecular Sequence Data,Nucleotide Mapping,Organ Specificity,RNA, Messenger,Ribonucleoproteins,Transcription, Genetic,Variation (Genetics),Heterogeneous-Nuclear Ribonucleoprotein Group A-B,Heterogeneous-Nuclear Ribonucleoproteins,RNA, Messenger,Ribonucleoproteins,hnRNP A1,DNA,DNA Restriction Enzymes
Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia, Italy.
Nucleic Acids Res.
0305-1048
0
False
2836799
