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Heterologous expression in Bacillus subt ... cloned hisG gene in B subtilis
Heterologous expression in Bacillus subtilis II In vitro removal of the attenuator sequence of the Escherichia coli his operon allows expression of the cloned hisG gene in B subtilis
5161
The promoter-proximal region of the Escherichia coli histidine (his) operon, including the promoter, the attenuator and the hisG gene, as well as the first of the nine structural genes of the his operon, have been cloned in Bacillus subtilis. In this host, the hisG gene could not be expressed because its transcription appeared to be irreversibly terminated at the attenuator (Ferretti et al., 1984). When the attenuator plus various lengths of the two bordering regions were removed, one of the attenuatorless sequences cloned in B. subtilis allowed the progression of transcription and complementation of the corresponding hisA mutation in this Gram-positive host. The deletion removed a 349-bp segment which contained the his attenuator and promoter. In B. subtilis, the productive transcription of the hisG gene started at a site in pAT153 and terminated in pC194. Sequence analysis of the deletion indicates that the E. coli ribosome-binding site of the his operon was used for the translation of the E. coli hisG gene mRNA in B. subtilis cells, which can thus grow in the absence of histidine.
Buvoli M, Damiani G, Ferretti L, Testori A, Sgaramella V
Gene
1986-01-01 00:00
47
2
279-86
Bacillus subtilis,Cloning, Molecular,Escherichia coli,Gene Expression Regulation,Genes, Bacterial,Histidine,Operon,Plasmids,Promoter Regions (Genetics),Transcription, Genetic,Histidine
Gene
0378-1119
0
False
3104143
