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Functional crosstalk between exon enhanc ... racts and branchpoint sequences
Functional crosstalk between exon enhancers, polypyrimidine tracts and branchpoint sequences
5161
We recently identified enhancer elements that activate the weak 3' splice site of alpha-tropomyosin exon 2 as well as a variety of heterologous weak 3' splice sites. To understand their mechanism of action, we devised an iterative selection strategy to identify functional pyrimidine tracts and branchpoint sequences in the presence or absence of enhancer elements. Surprisingly, we found that strong pyrimidine tracts were selected regardless of the presence of enhancer elements. However, the presence of enhancer elements resulted in the selection of multiple, non-consensus branchpoint sequences. Thus, enhancer elements apparently activate weak 3' splice sites primarily by increasing the efficiency of splicing of introns containing branchpoint sequences with less than optimal U2-branchpoint pairing arrangements. Comparison of consensus sequences from both our selection strategy and compilations of published intron sequences suggests that exon enhancer elements could be widespread and play an important role in the selection of 3' splice sites.
Buvoli M, Mayer SA, Patton JG
The EMBO journal
1997-12-01 00:00
16
23
7174-83
Animals,Exons,Models, Genetic,Nucleic Acid Conformation,Pyrimidines,RNA Splicing,RNA, Small Nuclear,Rats,Selection (Genetics),Tropomyosin,Pyrimidines,RNA, Small Nuclear,Tropomyosin
Department of Molecular Biology, Vanderbilt University, Box 1820 Station B, Nashville, TN 37235, USA.
EMBO J.
NIGMS GM50418
0261-4189
10.1093/emboj/16.23.7174
0
False
9384594
