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Activated type I phosphatidylinositol ki ... eptor following EGF stimulation
Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor EGF receptor following EGF stimulation
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We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at approximately 1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic detergent, indicated that the PI kinase described here was type I or PI 3' kinase. These results demonstrate the regulation of a type I PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway.
Bjorge JD, Chan TO, Antczak M, Kung HJ, Fujita DJ
Proceedings of the National Academy of Sciences of the United States of America
1990-05-01 00:00
87
10
3816-20
1-Phosphatidylinositol 4-Kinase,Animals,Antibodies,Cell Line,Enzyme Activation,Epidermal Growth Factor,Humans,Immunoblotting,Isoenzymes,Kinetics,Phosphotransferases,Receptor, Epidermal Growth Factor,Transfection,Antibodies,Isoenzymes,Epidermal Growth Factor,Phosphotransferases,Receptor, Epidermal Growth Factor,1-Phosphatidylinositol 4-Kinase
Department of Medical Biochemistry, University of Calgary, Canada.
Proc. Natl. Acad. Sci. U.S.A.
NCI CA39207
0027-8424
0
False
2160078
