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Osmotic stress sensitizes naturally resi ... to TNF-alpha-induced apoptosis
Osmotic stress sensitizes naturally resistant cells to TNF-alpha-induced apoptosis
7350
Most cells are naturally resistant to TNF-alpha-induced cell death and become sensitized when NF-kappaB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNF-alpha-induced cell death. We found that it sensitizes the naturally resistant HeLa cells to TNF-alpha-induced apoptosis, with the involvement of an increase in the activity of several kinases, the inhibition of Bcl-2 expression, and a late increase on NF-kappaB activation. Cell death occurs regardless of the enhanced NF-kappaB activity, whose inhibition produces an increase in apoptosis. The inhibition of p38 kinase, also involved in NF-kappaB activation, significantly increases the effect of osmotic stress on TNF-alpha-induced cell death.
Franco DL, Nojek IM, Molinero L, Coso OA, Costas MA
Cell death and differentiation
2002-10-01 00:00
9
10
1090-8
Apoptosis,Dose-Response Relationship, Drug,Enzyme Inhibitors,Eukaryotic Cells,Hela Cells,Humans,MAP Kinase Signaling System,Mitogen-Activated Protein Kinase 12,Mitogen-Activated Protein Kinase 8,Mitogen-Activated Protein Kinases,NF-kappa B,Osmotic Pressure,Proto-Oncogene Proteins c-bcl-2,Saline Solution, Hypertonic,Stress,Sulfasalazine,Transcription Factor RelA,Tumor Necrosis Factor-alpha,p38 Mitogen-Activated Protein Kinases,Enzyme Inhibitors,NF-kappa B,Proto-Oncogene Proteins c-bcl-2,Saline Solution, Hypertonic,Transcription Factor RelA,Tumor Necrosis Factor-alpha,Sulfasalazine,Mitogen-Activated Protein Kinase 12,Mitogen-Activated Protein Kinase 8,Mitogen-Activated Protein Kinases,p38 Mitogen-Activated Protein Kinases
Laboratorio de Fisiología y Biología Molecular, Departamento de Cs. Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, C1428EHA Bs. As., Argentina.
Cell Death Differ.
1350-9047
10.1038/sj.cdd.4401074
0
False
12232797
