Document Actions
My CV
short version. Updated June, 2007.
|
Department of Molecular, Cellular and Developmental Biology • University of Colorado • Boulder, CO 80309. |
|
Phone (303) 492-6725 • Fax (303) 492-1587 • E-mail hugo.olguin@colorado.edu |
Hugo C. Olguin M.
BORN: September 12, 1974 (Santiago, CHILE)
MARITAL STATUS: Married. One daughter.
|
Current research summary |
||
|
|
Postnatal growth and regeneration of skeletal muscle requires a population of resident myogenic precursors named satellite cells. The transcription factor Pax7 is critical for satellite cell biogenesis and survival and has been also implicated in satellite cell self renewal; however, the underlying molecular mechanisms remain unclear. Previously, I showed that Pax7 overexpression in adult primary myoblasts down-regulates MyoD and prevents myogenin induction, inhibiting myogenesis. Recently, I have shown that Pax7 prevents muscle differentiation independently of its transcriptional activity, affecting MyoD function. Conversely, myogenin directly affects Pax7 expression and may be critical for Pax7 down-regulation in differentiating cells. These results provide evidence for a cross-inhibitory interaction between Pax7 and members of the muscle regulatory factor family. This could represent an additional mechanism for the control of satellite cell fate decisions resulting in proliferation, differentiation, and self-renewal, necessary for skeletal muscle maintenance and repair. My current efforts are directed to the development of tools for (1) the unbiased identification of Pax7-interacting partners in myogenic cells, and (2) for the in vivo study of Pax7 function during muscle regeneration. |
|
|
Education |
||
|
|
1997-2002 P. Catholic University of Chile Santiago, Chile. Ph.D. Cellular and Molecular Biology 1992-1996 P. Catholic University of Chile Santiago, Chile. B.S. Biology |
|
|
research experience |
||
|
|
2002-present University of Colorado Boulder, CO Research Associate (Postdoc) Advisor: Dr. Bradley B. Olwin. Department of Molecular, Cellular and Developmental Biology. Research focus: Molecular basis for Pax7 function in satellite cell renewal 1999-2002 P. Catholic University of Chile Santiago, CHILE Graduate student research Advisor: Dr. Enrique Brandan. Department of Cellular and Molecular Biology. Research focus: Proteoglycans and the regulation of embryonic myogenesis. 1996-1997 P. Catholic University of Chile Santiago, CHILE DIPUC research assistant Advisor: Dr. Enrique Brandan. Department of Cellular and Molecular Biology. Research focus: Identification of proteoglycan ligands during myogenesis 1995-1996 P. Catholic University of Chile Santiago, CHILE Undergraduate student research Advisor: Dr. Enrique Brandan. Department of Cellular and Molecular Biology. Research focus: Proteoglycan expression during myogenesis and identification of proteoglycan ligands. |
|
|
publications |
||
|
|
Pisconti, A., Olguin, H.C., and Bradley B Olwin. Syndecan-3 regulates Notch signaling and stem cell fate. Manuscript in preparation. Olguin, H.C., and Olwin, B.B. Pax7-FHKR and MyoD functional interaction in alveolar rhabdomyosarcoma. Manuscript in preparation. Olguin, H.C., Yang, Z., Tapscott, S.J., and Olwin, B.B. (2007). Reciprocal inhibition between Pax7 and MRFs modulates myogenic cell fate determination. Journal Cell Biology. 177: 769-779. Olguin, H.C., and Olwin, B.B. (2004). Pax-7 up-regulation inhibits myogenesis and cell cycle progression to promote satellite cell quiescence. Developmental Biology. 275: 375-388. Casar, JC., Cabello-Verrugio, C., Olguin, H., Aldunate, R., Inestrosa, NC., and Brandan, E. (2004). Increased Expression of Heparan Sulfate Proteoglycans in Skeletal Muscle Regeneration: Relevance of Syndecan-3. Journal Cell Science. 117: 73-84. Olguin, H. Santander, C., and Brandan, E. (2003). Inhibition of myoblast migration, via decorin expression, is critical for normal skeletal muscle differentiation. Developmental Biology. 259: 209-224. Olguin, H. and Brandan, E. (2001). Expression and localization of proteoglycans during limb myogenic activation. Developmental Dynamics. 221:106-115. |
|
|
International Meetings |
||
|
|
Olguin, H.C., Yang, Z., Tapscott, S.J., and Olwin, B.B. Reciprocal inhibition between Pax7 and MRFs modulates myogenic cell fate determination. (Poster). Gordon Research Conference in Myogenesis. Il Ciocco, Italy. May 13-18, 2004. Olguin, H.C. and Olwin, B.B. Satellite cell self-renewal: ups and downs on the read. (Awarded Poster). Frontier in myogenesis (Society for Muscle Biology). Pine Mountain, Georgia (USA). April 27- May 2, 2006. Olguin, H.C. and Olwin, B.B. Identifying the molecular basis for Pax7 requirement in satellite cell renewal. (Speaker - selected poster). FASEB Summer Research Conferences: Skeletal Muscle and Satellite Cells. Tucson, Arizona (USA). June 11-16, 2005. Olguin, H.C. and Olwin, B.B. An essential role for Pax-7 in satellite cell quiescence. (Poster). Gordon Research Conference in Myogenesis. Il Ciocco, Italy. May 16-25, 2004. Olguin, H.C. and Olwin, B.B. Functional analysis of Pax-7 expression in satellite cells. (Poster). FASEB Summer Research Conferences: Skeletal Muscle and Satellite Cells. Tucson, Arizona (USA). July 26-31, 2003. Brandan, E., Casar, J. and Olguin, H. Expression of proteoglycans during skeletal muscle formation. 2001 Meeting of International Research Scholars, Howard Hughes Medical Institute. Vancouver, Canada. June 20-23, 2001. Olguin, H. and Brandan, E. Syndecan-3 and decorin antisense myoblast transplantation: effects of proteoglycan expression ablation on embryonic myogenesis in vivo. (Poster). 27th Meeting of the Federation of European Biochemical Societies. Lisbon, Portugal. June 30–July 5, 2001 Olguin, H. and Brandan, E. Decorin and Syndecan-3 expression are upregulated during early limb myogenesis. (Poster). Molecular Biology of Muscle Development and Disease. Pacific Grove, California (USA). May 21-26, 2000. Olguin, H. and Brandan, E. Characterization of proteoglycans expression during early mouse limb myogenesis. (Poster). 39th American Society for Cell Biology Annual Meeting Washington, DC (USA). December 11-15, 1999. Olguin, H., Larraín, J., and Brandan, E. Expression of proteoglycans during limb formation in vivo and in vitro and activation of myogenic program. (Poster). 5th Brazilian Symposium of Extracellular Matrix (Simec 98). Angra do Rais, Brasil. September 7-10, 1998. |
|
|
Awards |
||
|
|
Lymn Foundation New Investigator Awards at the Frontier in myogenesis Meeting (Society for Muscle Biology). Pine Mountain, Georgia (USA). April 27- May 2, 2006. Graduated Summa Cum Laude, PhD in Cellular and Molecular Biology, P. Catholic University of Chile, 2002. P. Catholic University of Chile Fellowship for external scientific training. Visited laboratory: Dr. Delphine Duprez. Institute of Cellular and Molecular Embryology (CNRS). Nogent-sur-Marne. France (January 30th to April 30th, 2000)National Committee on Science and Technology (CONICYT-Chile) Graduate Student Fellowship, 1997-2001. Best Graduated Student Excellence Award, Bachelors in Biological Sciences, P. Catholic University of Chile, 1996. Chilean Education Ministry (MINEDUC) Fellowship for undergraduate students, 1992-1995. P. Catholic University of Chile Fellowship for undergraduate students, 1992-1995. |
|
|
Grants |
||
|
|
Muscular Dystrophy Association (MDA3928), Principal Investigator. “Pax7 role in satellite cell self-renewal”. 2005-2007. National Fund for Science and Technology Development (FONDECYT) (Graduate Student Section # 2990086), Principal Investigator. “Mechanisms that activate skeletal muscle differentiation: proteoglycans and the regulation of embryonic myogenesis”. 1999-2001. |
|
|
Professional memberships |
||
|
|
Latin American Society of Developmental Biology. |
|
|
Skills |
||
|
§ DNA & RNA: molecular cloning, plasmid preparation, genomic DNA isolation, southern blot, PCR, PCR based gene mutagenesis. Construction and purification of recombinant adenoviruses. Quantitative RT-PCR, northern blots, in situ hybridization. § Proteins: SDS-PAGE, ND-PAGE, kinase assays, general chromatography techniques, radioactive metabolic labeling of cells (3H, 35S, 32P), pulse-chase, western blot, Tandem affinity purification, reporter gene assays, construction and expression of recombinant proteins. § Immunological techniques: immuno and co-immuno-purification, chromatin immuno-precipitation, immunoprecipitation, western blotting, immunofluorescence, immuno-histochemistry, fluorescent-activated cell sorting (FACS) § Cell and tissue culture: primary (mouse skeletal muscle; embryonic and adult) and cell lines (e.g. C2C12, MM14, C3H/10T1/2, 293 and derivates), isolated skeletal muscle myofiber preparation and culture, transient and stable transfection, adenoviral and retroviral infection. § Animal models: mouse (embryo and adult. In adult muscle: induced degeneration and regeneration; cell engraftment). Chicken (embryo: cell engraftment, microinjection, retroviral infection). § Histology and microscopy: tissue dissection and sectioning (frozen and paraffin); general techniques for dissection and fluorescent microscopy. References |
||
|
|
Dr. Bradley B. Olwin Department of Molecular, Cellular and Developmental Biology 347 UCB. University of Colorado Boulder, CO 80309 Phone: (303) 492 6816 Fax: (303) 492 1587 e-mai: Bradley.Olwin@colorado.edu Dr. Leslie A. Leinwand Department of Molecular, Cellular and Developmental Biology 347 UCB. University of Colorado Boulder, CO 80309 Phone: (303) 492 7606 Fax: (303) 492 8907 e-mai: Leslie.Leinwand@colorado.edu
Dr. Enrique Brandan Departamento de Biología Celular y Molecular. Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. Alameda # 340. Santiago. PO Box 114-D. Santiago, Chile. Phone: 56-2-6862724 Fax: 56-2-6355395 e-mail: ebrandan@ bio.puc.cl |
|
|
|
|
|
