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Versatile 5 phosphoryl coupling of small and large molecules to an RNA
Versatile 5' phosphoryl coupling of small and large molecules to an RNA.
47
A Ca2+-requiring catalytic RNA is shown to create 5' phosphate-phosphate linkages with all nucleotides and coenzymes including CoA, nicotinamide adenine dinucleotide phosphate, thiamine phosphate, thiamine pyrophosphate, and flavin mononucleotide. In addition to these small molecules, macromolecules such as RNAs with 5'-diphosphates, and nonnucleotide molecules like Nepsilon-phosphate arginine and 6-phosphate gluconic acid also react. That is, the self-capping RNA isolate 6 is an apparently universal 5' phosphate-linker, reacting with any nucleophile containing an unblocked phosphate. These RNA reactions demonstrate a unique RNA catalytic capability and imply versatile and specific posttranscriptional RNA modification by RNA catalysis.
Huang F, Yarus M
Proceedings of the National Academy of Sciences of the United States of America
1997-08-19 00:00
94
17
8965-9
Calcium,Catalysis,Guanosine Diphosphate,Phosphates,RNA,RNA Processing, Post-Transcriptional,Phosphates,Guanosine Diphosphate,RNA,Calcium
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM30881, NIGMS GM48080
0027-8424
1497
True
9256418
