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Three small ribooligonucleotides with specific arginine sites
Three small ribooligonucleotides with specific arginine sites.
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Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge loops, whose sequences can include arginine coding triplets. Binding is highly specific for arginine, but all three motifs, like the self-splicing group I intron, also bind guanosine 5'-monophosphate. One site is stereoselective, somewhat preferring D-arginine.
Connell GJ, Illangesekare M, Yarus M
Biochemistry
1993-06-01 00:00
32
21
5497-502
Arginine,Base Sequence,Binding Sites,Kinetics,Molecular Sequence Data,Nucleic Acid Conformation,Oligoribonucleotides,Polymerase Chain Reaction,Structure-Activity Relationship,Oligoribonucleotides,Arginine
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347
Biochemistry
NIGMS GM 30881
0006-2960
1487
True
8504070
