Document Actions
Suppression of eukaryotic translation termination by selected RNAs
Suppression of eukaryotic translation termination by selected RNAs.
47
Using selection-amplification, we have isolated RNAs with affinity for translation termination factors eRF1 and eRF1.eRF3 complex. Individual RNAs not only bind, but inhibit eRF1-mediated release of a model nascent chain from eukaryotic ribosomes. There is also significant but weaker inhibition of eRF1-stimulated eRF3 GTPase and eRF3 stimulation of eRF1 release activity. These latter selected RNAs therefore hinder eRF1.eRF3 interactions. Finally, four RNA inhibitors of release suppress a UAG stop codon in mammalian extracts dependent for termination on eRF1 from several metazoan species. These RNAs are therefore new specific inhibitors for the analysis of eukaryotic termination, and potentially a new class of omnipotent termination suppressors with possible therapeutic significance.
Carnes J, Frolova L, Zinnen S, Drugeon G, Phillippe M, Justesen J, Haenni AL, Leinwand L, Kisselev LL, Yarus M
RNA (New York, N.Y.)
2000-10-01 00:00
6
10
1468-79
Animals,Base Sequence,Capsid,Chromatography, Thin Layer,Codon, Terminator,GTP Phosphohydrolases,Humans,Molecular Mimicry,Nucleic Acid Conformation,Peptide Chain Termination, Translational,Peptide Termination Factors,Protein Binding,RNA,RNA Stability,RNA, Messenger,RNA, Viral,Templates, Genetic,Thermodynamics,Xenopus Proteins,Xenopus laevis,Codon, Terminator,ETF1 protein, human,Peptide Termination Factors,RNA, Messenger,RNA, Viral,SUP45 protein, Xenopus,Xenopus Proteins,peptide chain-termination release factor 1,peptide-chain-release factor 3,RNA,GTP Phosphohydrolases
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347, USA
RNA
NIGMS GM 30881, NIGMS GM 48080, NHLBI HL65610
1355-8382
1474
True
11073222
