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RNA-catalyzed amino acid activation
RNA-catalyzed amino acid activation.
47
We have selected RNAs that perform a new reaction that chemically activates amino acids, paralleling mixed phosphate anhydride synthesis by protein aminoacyl-transfer RNA synthetases. Care with recovery of the unstable reaction product was apparently essential to this selection. The best characterized RNA, KK13, requires only Ca2+ for reaction and is optimally active at low pH with KM = 50 mM and kcat = 1.1 min(-1) for activation of leucine. In conjunction with previous RNA-catalyzed aminoacyl-RNA synthesis, peptide bond formation, and RNA-based coding, these amino acid-activating RNAs complete an experimental demonstration that the four fundamental reactions of protein biosynthesis can be RNA-mediated. The appearance of translation in an RNA world is therefore supported.
Kumar RK, Yarus M
Biochemistry
2001-06-19 00:00
40
24
6998-7004
5'-Guanylic Acid,Amino Acids,Aminoacylation,Base Sequence,Carboxylic Acids,Catalysis,Chromatography, High Pressure Liquid,Cloning, Molecular,Diphosphates,Guanosine Triphosphate,Leucine,Molecular Sequence Data,Nucleic Acid Conformation,RNA Caps,RNA, Catalytic,Amino Acids,Carboxylic Acids,Diphosphates,RNA Caps,RNA, Catalytic,Leucine,5'-Guanylic Acid,Guanosine Triphosphate
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
Biochemistry
NIGMS GM 30881
0006-2960
bi010710x
1451
True
11401543
