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IsoleucineRNA sites with associated coding sequences
Isoleucine:RNA sites with associated coding sequences.
47
An RNA family that binds isoleucine with Kd = 200-500 microM was repetitively isolated from a ribonucleotide transcript pool containing 50 randomized positions. The RNA site is specific, discriminating against branched side chains of different size (valine--one methylene smaller than isoleucine) by at least 1.3 kcal/mol and against the shape (linear) of norleucine by 0.6 kcal/mol. The binding site was localized by sequence comparison, by synthesis of mutant and truncated active derivatives, and by chemical modification-interference. The binding site is small, no more than 10-12 nt, containing an asymmetric internal loop (6 over 2 nt) that includes the isoleucine codon AUU and a sequence of four G's, two of which are involved in G-U and G-C base pairs. Areas of U/G concentration like these may signal a hydrophobic RNA site.
Majerfeld I, Yarus M
RNA (New York, N.Y.)
1998-04-01 00:00
4
4
471-8
Amides,Amino Acids,Base Sequence,Binding Sites,Chromatography, Affinity,Isoleucine,Lead,Molecular Sequence Data,Nucleic Acid Conformation,RNA,Sequence Analysis, RNA,Zinc,Amides,Amino Acids,RNA,Isoleucine,Lead,Zinc
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347, USA
RNA
1355-8382
1430
True
9630252
