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An RNA pocket for an aliphatic hydrophobe
An RNA pocket for an aliphatic hydrophobe.
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We have isolated an RNA with specific affinity for the L-valine side chain, using selection-amplification. The active RNA secondary structure, identified by repeated selection, is a highly conserved asymmetric (4:10) internal loop adjacent to required G-U pairs. The binding free-energy per methylene is up to 1.5 kcal mol-1, and very dependent on group position. Amino acid binding is L-stereoselective and distinguishes aliphatic sidechains by size and, given the same total size, by configuration. Though aliphatic-RNA interactions have frequently been neglected, their avidity and specificity seem sufficient for a biological role.
Majerfeld I, Yarus M
Nature structural biology
1994-05-01 00:00
1
5
287-92
Amino Acids,Base Sequence,Binding Sites,Chemistry, Physical,Chromatography, Affinity,Kinetics,Magnesium,Molecular Sequence Data,Nucleic Acid Conformation,RNA,Selection (Genetics),Stereoisomerism,Substrate Specificity,Valine,Amino Acids,RNA,Valine,Magnesium
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347, USA
Nat. Struct. Biol.
1072-8368
1399
True
7545073
