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A more complex isoleucine aptamer with a cognate triplet
A more complex isoleucine aptamer with a cognate triplet.
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The simplest RNA that can meet a column affinity selection for isoleucine was previously defined using selection amplification with decreasing numbers of randomized nucleotides. This simplest UAUU motif was a small asymmetric internal loop. Conserved positions of the loop include isoleucine codon and anticodon triplets (Lozupone C., Changayil, S., Majerfeld, I., and Yarus, M. (2003) RNA (N. Y.) 9, 1315-1322). Using new primer sequences, we now select a somewhat more complex isoleucine binding RNA, requiring 4.7 more bits of information to describe. The newly selected structure is a terminal or hairpin loop of 20 nucleotides, 15 being invariant. An information profile shows that the new binding site contains five short functional loop regions joined by less significant single nucleotide positions. Among the important nucleotides is a conserved isoleucine anticodon, supporting the escaped triplet theory, which posits a stereochemical genetic code originating in RNA amino acid binding sites.
Legiewicz M, Yarus M
The Journal of biological chemistry
2005-05-20 00:00
280
20
19815-22
Anticodon,Base Sequence,Binding Sites,Chromatography, Affinity,Codon,Isoleucine,Nucleic Acid Conformation,Oligoribonucleotides,RNA,Anticodon,Codon,Oligoribonucleotides,RNA,Isoleucine
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, USA
J. Biol. Chem.
NIGMS GM 48080
0021-9258
10.1074/jbc.M502329200
M502329200
1387
True
15772067
