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5-RNA self-capping from guanosine diphosphate
5'-RNA self-capping from guanosine diphosphate.
47
A selected RNA (isolate 6) efficiently catalyzes a self-capping reaction with free GDP, yielding the same 5'-capped structure as is formed by protein GTP:RNA guanylyltransferase. This unexplored RNA-catalyzed reaction type involving nucleophilic attack on phosphate by phosphate adds to the variety of possible postsynthetic RNA-catalyzed RNA modifications. The selected RNA requires only Ca2+ for activation and has a broad active pH range of 4.5-9.0. The RNA also has a 5'-pyrophosphatase activity.
Huang F, Yarus M
Biochemistry
1997-06-03 00:00
36
22
6557-63
Base Sequence,Calcium,Guanosine Diphosphate,Guanosine Triphosphate,Hydrogen-Ion Concentration,Hydrolysis,Molecular Sequence Data,Nucleotidyltransferases,Pyrophosphatases,RNA,RNA Caps,Ribonucleases,Sodium Hydroxide,RNA Caps,RNA, circular,Sodium Hydroxide,Guanosine Diphosphate,RNA,Calcium,Guanosine Triphosphate,Nucleotidyltransferases,mRNA guanylyltransferase,Ribonucleases,Pyrophosphatases
Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, 80309-0347, USA
Biochemistry
NIGMS GM30881, NIGMS GM48080
0006-2960
10.1021/bi970475b
bi970475b
1384
True
9184134
