Document Actions
Isolation and characterization of a nema ... ment from Panagrellus redivivus
Isolation and characterization of a nematode transposable element from Panagrellus redivivus.
45
We have isolated a transposable element, designated PAT-1, from the free-living nematode Panagrellus redivivus. P. redivivus strain C15 was found to have a high spontaneous mutation frequency compared to the standard Caenorhabditis elegans laboratory strain N2. To characterize the genetic lesions occurring in spontaneous C15 mutants, we molecularly cloned the homolog of the C. elegans unc-22 gene from wild-type P. redivivus and two strains carrying spontaneous mutations in this gene. One of these mutations resulted from the insertion of a 4.8-kilobase segment of repetitive DNA. This repetitive element (PAT-1) varies in copy number (10-50 copies) and location in different P. redivivus strains and is absent from C. elegans. The element could be useful as a transformation vector for C. elegans. Our approach is a general one that could be used to isolate additional nematode transposons from other species.
Link CD, Graf-Whitsel J, Wood WB
Proceedings of the National Academy of Sciences of the United States of America
1987-08-01 00:00
84
15
5325-9
Animals,Caenorhabditis,Cloning, Molecular,DNA Restriction Enzymes,DNA Transposable Elements,Mutation,Nematoda,Polymorphism, Genetic,DNA Transposable Elements,DNA Restriction Enzymes
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM-08668, NICHD HD-11762
0027-8424
1333
True
3037542
