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Immunofluorescence visualization of germ ... dults of Caenorhabditis elegans
Immunofluorescence visualization of germ-line-specific cytoplasmic granules in embryos, larvae, and adults of Caenorhabditis elegans.
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By using fluorescent antibody staining, we have followed cytoplasmic granules unique to germ-line cells throughout the life cycle of Caenorhabditis elegans. These elements, designated P granules, are segregated exclusively to germ-line precursor cells during early embryogenesis. Prior to mitosis at each of the early cleavages that produce a somatic and germ-line daughter cell, the granules become localized in the region of cytoplasm destined for the germ-line daughter. After the 16-cell stage, the granules appear to be associated with the nuclear envelope. P granules persist in the germ cells throughout the larval and adult stages. The P granules are similar in number, size, and distribution to germ-line-specific structures identified as germinal plasm by electron microscopy in C. elegans embryos.
Strome S, Wood WB
Proceedings of the National Academy of Sciences of the United States of America
1982-03-01 00:00
79
5
1558-62
Animals,Caenorhabditis,Cell Compartmentation,Cytoplasmic Granules,Fluorescent Antibody Technique,Gonads
Proc. Natl. Acad. Sci. U.S.A.
NICHD F32-HD05845, NICHD HD11762, NICHD HD14958
0027-8424
1331
True
7041123
