Document Actions
Caenorhabditis elegans orthologs of the ... n receptor nuclear translocator
Caenorhabditis elegans orthologs of the aryl hydrocarbon receptor and its heterodimerization partner the aryl hydrocarbon receptor nuclear translocator.
45
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor, until now described only in vertebrates, that mediates many of the carcinogenic and teratogenic effects of certain environmental pollutants. Here, we describe orthologs of AHR and its dimerization partner AHR nuclear translocator (ARNT) in the nematode Caenorhabditis elegans, encoded by the genes ahr-1 and aha-1, respectively. The corresponding proteins, AHR-1 and AHA-1, share biochemical properties with their mammalian cognates. Specifically, AHR-1 forms a tight association with HSP90, and AHR-1 and AHA-1 interact to bind DNA fragments containing the mammalian xenobiotic response element with sequence specificity. Yeast expression studies indicate that C. elegans AHR-1, like vertebrate AHR, requires some form of post-translational activation. Moreover, this requirement depends on the presence of the domains predicted to mediate binding of HSP90 and ligand. Preliminary experiments suggest that if AHR-1 is ligand-activated, its spectrum of ligands is different from that of the mammalian receptor: C. elegans AHR-1 is not photoaffinity labeled by a dioxin analog, and it is not activated by beta-naphthoflavone in the yeast system. The discovery of these genes in a simple, genetically tractable invertebrate should allow elucidation of AHR-1 function and identification of its endogenous regulators.
Powell-Coffman JA, Bradfield CA, Wood WB
Proceedings of the National Academy of Sciences of the United States of America
1998-03-17 00:00
95
6
2844-9
Amino Acid Sequence,Animals,Aryl Hydrocarbon Receptor Nuclear Translocator,Caenorhabditis elegans,Caenorhabditis elegans Proteins,DNA-Binding Proteins,Dimerization,Dioxins,Evolution, Molecular,Genes, Helminth,HSP90 Heat-Shock Proteins,Mice,Molecular Sequence Data,Protein Binding,Receptors, Aryl Hydrocarbon,Regulatory Sequences, Nucleic Acid,Sequence Homology, Amino Acid,Species Specificity,Transcription Factors,Xenobiotics,Ahr protein, mouse,Arnt protein, mouse,Caenorhabditis elegans Proteins,DNA-Binding Proteins,Dioxins,HSP90 Heat-Shock Proteins,Receptors, Aryl Hydrocarbon,Transcription Factors,Xenobiotics,ahr-1 protein, C elegans,Aryl Hydrocarbon Receptor Nuclear Translocator
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Proc. Natl. Acad. Sci. U.S.A.
NIEHS ES-05703, NIGMS GM-16294, NICHD HD-11762
0027-8424
1309
True
9501178
