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Saccharomyces cerevisiae MPS2 encodes a ... e body and the nuclear envelope
Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope.
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The MPS2 (monopolar spindle two) gene is one of several genes required for the proper execution of spindle pole body (SPB) duplication in the budding yeast Saccharomyces cerevisiae (). We report here that the MPS2 gene encodes an essential 44-kDa protein with two putative coiled-coil regions and a hydrophobic sequence. Although MPS2 is required for normal mitotic growth, some null strains can survive; these survivors exhibit slow growth and abnormal ploidy. The MPS2 protein was tagged with nine copies of the myc epitope, and biochemical fractionation experiments show that it is an integral membrane protein. Visualization of a green fluorescent protein (GFP) Mps2p fusion protein in living cells and indirect immunofluorescence microscopy of 9xmyc-Mps2p revealed a perinuclear localization with one or two brighter foci of staining corresponding to the SPB. Additionally, immunoelectron microscopy shows that GFP-Mps2p localizes to the SPB. Our analysis suggests that Mps2p is required as a component of the SPB for insertion of the nascent SPB into the nuclear envelope.
Muñoz-Centeno MC, McBratney S, Monterrosa A, Byers B, Mann C, Winey M
Molecular biology of the cell
1999-07-01 00:00
10
7
2393-406
Adenosine Triphosphatases,Amino Acid Sequence,Cell Cycle,DNA-Binding Proteins,Fungal Proteins,Membrane Proteins,Mitotic Spindle Apparatus,Molecular Sequence Data,Nuclear Envelope,Nuclear Proteins,Recombinant Proteins,Saccharomyces cerevisiae,Saccharomyces cerevisiae Proteins,DNA-Binding Proteins,Fungal Proteins,MPS2 protein, S cerevisiae,Membrane Proteins,Nuclear Proteins,Recombinant Proteins,Saccharomyces cerevisiae Proteins,Adenosine Triphosphatases,RPT1 protein, S cerevisiae
Service de Biochimie et de Gntique Molculaire, Commissariat lEnergie AtomiqueSaclay, F-91191 Gif-sur-Yvette, France
Mol. Biol. Cell
NIGMS GM-18473
1059-1524
1274
True
10397772
