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New alleles of the yeast MPS1 gene revea ... n spindle pole body duplication
New alleles of the yeast MPS1 gene reveal multiple requirements in spindle pole body duplication.
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In Saccharomyces cerevisiae, the Mps1p protein kinase is critical for both spindle pole body (SPB) duplication and the mitotic spindle assembly checkpoint. The mps1-1 mutation causes failure early in SPB duplication, and because the spindle assembly checkpoint is also compromised, mps1-1 cells proceed with a monopolar mitosis and rapidly lose viability. Here we report the genetic and molecular characterization of mps1-1 and five new temperature-sensitive alleles of MPS1. Each of the six alleles contains a single point mutation in the region of the gene encoding the protein kinase domain. The mutations affect several residues conserved among protein kinases, most notably the invariant glutamate in subdomain III. In vivo and in vitro kinase activity of the six epitope-tagged mutant proteins varies widely. Only two display appreciable in vitro activity, and interestingly, this activity is not thermolabile under the assay conditions used. While five of the six alleles cause SPB duplication to fail early, yielding cells with a single SPB, mps1-737 cells proceed into SPB duplication and assemble a second SPB that is structurally defective. This phenotype, together with the observation of intragenic complementation between this unique allele and two others, suggests that Mps1p is required for multiple events in SPB duplication.
Schutz AR, Winey M
Molecular biology of the cell
1998-04-01 00:00
9
4
759-74
Alleles,Amino Acid Sequence,Conserved Sequence,Epitopes,Mitotic Spindle Apparatus,Molecular Sequence Data,Mutation,Phenotype,Protein Kinases,Protein-Serine-Threonine Kinases,Protein-Tyrosine Kinases,Recombinant Fusion Proteins,Saccharomyces cerevisiae,Saccharomyces cerevisiae Proteins,Epitopes,Recombinant Fusion Proteins,Saccharomyces cerevisiae Proteins,MPS1 protein, S cerevisiae,Protein-Tyrosine Kinases,Protein Kinases,Protein-Serine-Threonine Kinases
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
Mol. Biol. Cell
NIGMS GM-07135, NIGMS GM-51312
1059-1524
1267
True
9529376
