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Multi-step control of spindle pole body duplication by cyclin-dependent kinase
Multi-step control of spindle pole body duplication by cyclin-dependent kinase.
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Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.
Haase SB, Winey M, Reed SI
Nature cell biology
2001-01-01 00:00
3
1
38-42
Cell Cycle,Cell Transformation, Neoplastic,Centrosome,Cyclin B,Cyclin-Dependent Kinases,Cyclins,Mitosis,Mitotic Spindle Apparatus,Saccharomyces cerevisiae Proteins,Yeasts,CLB6 protein, S cerevisiae,Cyclin B,Cyclins,Saccharomyces cerevisiae Proteins,CLB5 protein, S cerevisiae,Cyclin-Dependent Kinases
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 94035, USA
Nat. Cell Biol.
NIGMS GM38328, NIGMS GM51312
1465-7392
10.1038/35050543
1262
True
11146624
