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Epsilon-tubulin is required for centriol ... on and microtubule organization
Epsilon-tubulin is required for centriole duplication and microtubule organization.
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Centrosomes nucleate microtubules and serve as poles of the mitotic spindle. Centrioles are a core component of centrosomes and duplicate once per cell cycle. We previously identified epsilon-tubulin as a new member of the tubulin superfamily that localizes asymmetrically to the two centrosomes after duplication. We show that recruitment of epsilon-tubulin to the new centrosome can only occur after exit from S phase and that epsilon-tubulin is associated with the sub-distal appendages of mature centrioles. Xenopus laevis epsilon-tubulin was cloned and shown to be similar to human epsilon-tubulin in both sequence and localization. Depletion of epsilon-tubulin from Xenopus egg extracts blocks centriole duplication in S phase and formation of organized centrosome-independent microtubule asters in M phase. We conclude that epsilon-tubulin is a component of the sub-distal appendages of the centriole, explaining its asymmetric localization to old and new centrosomes, and that epsilon-tubulin is required for centriole duplication and organization of the pericentriolar material.
Chang P, Giddings TH, Winey M, Stearns T
Nature cell biology
2003-01-01 00:00
5
1
71-6
Animals,Base Sequence,Cell Cycle,Cells, Cultured,Centrioles,Cytoskeletal Proteins,DNA Primers,Female,GTP-Binding Proteins,Leucine Zippers,Male,Microscopy, Immunoelectron,Microtubules,Nuclear Proteins,Ovum,Protein Isoforms,Ranidae,S Phase,Sperm-Ovum Interactions,Spermatozoa,Tubulin,Cytoskeletal Proteins,DNA Primers,NIN protein, human,Nuclear Proteins,Protein Isoforms,Tubulin,GTP-Binding Proteins
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
Nat. Cell Biol.
NIGMS GM 51312, NIGMS GM52022
1465-7392
10.1038/ncb900
ncb900
1250
True
12510196
