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AUUUA sequences direct mRNA deadenylatio ... uring Xenopus early development
AUUUA sequences direct mRNA deadenylation uncoupled from decay during Xenopus early development.
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To study the regulation of AUUUA-mediated RNA deadenylation and destabilization during Xenopus early development, we microinjected chimeric mRNAs containing Xenopus or mammalian 3' untranslated region (3'-UTR) sequences into Xenopus oocytes, mature eggs, or fertilized embryos. We found that the AU-rich elements (ARE) of Xenopus c-myc II and the human granulocyte-macrophage colony-stimulating factor gene (GMCSF) both direct deadenylation of chimeric mRNAs in an AUUUA-dependent manner. In the case of the Xenopus c-myc II ARE, mutation of a single AUUUA within an absolutely conserved 11-nucleotide region in c-myc 3'-UTRs prevents ARE-mediated deadenylation. AUUUA-specific deadenylation appears to be developmentally regulated: low deadenylation activity is observed in the oocyte, whereas rapid deadenylation occurs following egg activation or fertilization. Deadenylation results in the accumulation of stable deadenylated RNAs that become degraded only following mid-blastula transition. We conclude that ARE-mediated mRNA deadenylation can be uncoupled from ARE-mediated mRNA decay and that AUUUAs directly signal deadenylation during Xenopus early development.
Voeltz GK, Steitz JA
Molecular and cellular biology
1998-12-01 00:00
18
12
7537-45
3' Untranslated Regions,Animals,Conserved Sequence,Fertilization,Granulocyte-Macrophage Colony-Stimulating Factor,Microinjections,Oligodeoxyribonucleotides,Oocytes,Poly A,RNA Processing, Post-Transcriptional,RNA, Messenger,Xenopus,3' Untranslated Regions,Oligodeoxyribonucleotides,RNA, Messenger,Poly A,Granulocyte-Macrophage Colony-Stimulating Factor
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06536, USA
Mol. Cell. Biol.
NCI CA16038
0270-7306
1224
True
9819439
