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ATP requirement for Prp5p function is de ... ructure of U2 small nuclear RNA
ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA.
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Stable addition of U2 small nuclear ribonucleoprotein (snRNP) to form the prespliceosome is the first ATP-dependent step in splicing, and it requires the DEXD/H box ATPase Prp5p. However, prespliceosome formation occurs without ATP in extracts lacking the U2 snRNP protein Cus2p. Here we show that Prp5p is required for the ATP-independent prespliceosome assembly that occurs in the absence of Cus2p. Addition of recombinant Cus2p can restore the ATP dependence of prespliceosome assembly, but only if it is added before Prp5p. Prp5p with an altered ATP-binding domain (Prp5-GNTp) can support growth in vivo, but only in a cus2 deletion strain, mirroring the in vitro results. Other Prp5 ATP-binding domain substitutions are lethal, even in the cus2 deletion strain, but can be suppressed by U2 small nuclear RNA mutations that hyperstabilize U2 stem IIa. We infer that the presence of Cus2p and stem IIa-destabilized forms of U2 small nuclear RNA places high demands on the ATP-driven function of Prp5p. Because Prp5p is not dispensable in vitro even in the absence of ATP, we propose that the core Prp5p function in bringing U2 to the branchpoint is not directly ATP-dependent. The positive role of Cus2p in rescuing mutant U2 can be reconciled with its antagonistic effect on Prp5 function in a model whereby Cus2p first helps Prp5p to activate the U2 snRNP for prespliceosome formation but then is displaced by Prp5p before or during the stabilization of U2 at the branchpoint.
Perriman R, Barta I, Voeltz GK, Abelson J, Ares M
Proceedings of the National Academy of Sciences of the United States of America
2003-11-25 00:00
100
24
13857-62
Adenosine Triphosphate,Base Sequence,Binding Sites,DEAD-box RNA Helicases,Genes, Fungal,Models, Biological,Molecular Sequence Data,Mutation,Nucleic Acid Conformation,Protein Structure, Tertiary,RNA Helicases,RNA Splicing,RNA, Fungal,RNA, Small Nuclear,RNA-Binding Proteins,Recombinant Proteins,Ribonucleoproteins, Small Nuclear,Saccharomyces cerevisiae,Saccharomyces cerevisiae Proteins,Spliceosomes,Trans-Activators,CUS2 protein, S cerevisiae,RNA, Fungal,RNA, Small Nuclear,RNA-Binding Proteins,Recombinant Proteins,Ribonucleoproteins, Small Nuclear,Saccharomyces cerevisiae Proteins,Trans-Activators,U2 small nuclear RNA,Adenosine Triphosphate,DEAD-box RNA Helicases,RNA Helicases,PRP5 protein, S cerevisiae
Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM32637, NIGMS GM47408
0027-8424
10.1073/pnas.2036312100
2036312100
1223
True
14610285
