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A novel embryonic polyA binding protein, ... ylation in Xenopus egg extracts
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts.
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An in vitro system that recapitulates the in vivo effect of AU-rich elements (AREs) on mRNA deadenylation has been developed from Xenopus activated egg extracts. ARE-mediated deadenylation is uncoupled from mRNA body decay, and the rate of deadenylation increases with the number of tandem AUUUAs. A novel ARE-binding protein called ePAB (for embryonic poly(A)-binding protein) has been purified from this extract by ARE affinity selection. ePAB exhibits 72% identity to mammalian and Xenopus PABP1 and is the predominant poly(A)-binding protein expressed in the stage VI oocyte and during Xenopus early development. Immunodepletion of ePAB increases the rate of both ARE-mediated and default deadenylation in vitro. In contrast, addition of even a small excess of ePAB inhibits deadenylation, demonstrating that the ePAB concentration is critical for determining the rate of ARE-mediated deadenylation. These data argue that ePAB is the poly(A)-binding protein responsible for stabilization of poly(A) tails and is thus a potential regulator of mRNA deadenylation and translation during early development.
Voeltz GK, Ongkasuwan J, Standart N, Steitz JA
Genes & development
2001-03-15 00:00
15
6
774-88
Adenosine Monophosphate,Amino Acid Sequence,Animals,Base Sequence,Blotting, Western,DNA Methylation,Gene Expression Regulation, Developmental,Kinetics,Molecular Sequence Data,Oocytes,Plasmids,Poly A,Poly(A)-Binding Proteins,Precipitin Tests,Protein Binding,RNA-Binding Proteins,Recombinant Proteins,Sequence Analysis, DNA,Sequence Homology, Amino Acid,Time Factors,Trans-Activation (Genetics),Ultraviolet Rays,Xenopus,Xenopus Proteins,Poly(A)-Binding Proteins,RNA-Binding Proteins,Recombinant Proteins,Xenopus Proteins,ePAB protein, Xenopus,Poly A,Adenosine Monophosphate
Department of Molecular Biophysics and Biochemistry, Yale University, Howard Hughes Medical Institute, New Haven, Connecticut 06536, USA
Genes Dev.
NCI CA16038
0890-9369
10.1101/gad.872201
1222
True
11274061
