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Preimplantation human embryonic developm ... gs after in vitro fertilization
Preimplantation human embryonic development from polypronuclear eggs after in vitro fertilization.
43
The occurrence of grossly normal-appearing morula stage human embryos (8- to 12-cell) that developed from eggs fertilized in vitro containing three pronuclei is described. Serial section analysis by transmission electron microscopy demonstrated that 25% of the blastomeres were multinucleate, with as many as five deoxyribonucleic acid-containing nuclei (determined by fluorescence microscopy) in a single cell. Light and electron microscopy indicated that normal cortical and zona reactions had taken place. Fine-structural development of the cytoplasm was characteristic of morula stage human embryos. The results suggest the need to determine pronuclear number prior to syngamy and are discussed with respect to the notion that human embryos may have the capacity to develop normally in spite of the presence of abnormal cells.
Van Blerkom J, Henry G, Porreco R
Fertility and sterility
1984-05-01 00:00
41
5
686-96
Blastomeres,Cell Nucleus,Embryonic Development,Female,Fertilization in Vitro,Humans,Microscopy, Electron,Oocytes,Pregnancy
Fertil. Steril.
0015-0282
1206
True
6714447
