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Occurrence of maternal and paternal spin ... ects after silent fertilization
Occurrence of maternal and paternal spindles in unfertilized human oocytes: possible relationship to nucleation defects after silent fertilization.
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Experience with conventional clinical IVF indicates that a first cleavage can occur in the absence of detectable pronuclear formation (so-called silent fertilization). In these instances, the first division is often asymmetrical and delayed when compared with normally fertilized siblings. In this study, DNA configurations and spindle organization were examined by fluorescence microscopy in metaphase II human oocytes that remained unfertilized after conventional IVF and were considered likely candidates for silent fertilization. The results show comparatively high frequencies of penetration in the absence of detectable pronuclear evolution, and that both a maternal meiotic and a sperm-derived mitotic-like spindle can coexist in the same oocyte. Patterns of cell division and blastomere nucleation in silent fertilizations suggest the possibility that this division may involve uniparental chromosomal segregation in which maternal and paternal DNA is differentially partitioned into daughter blastomeres. This pattern of inheritance may generate certain types of ploidy and nucleation defects detected at the 2- to 4-cell stage.
Van Blerkom J, Davis P, Alexander S
Reproductive biomedicine online
2004-04-01 00:00
8
4
454-9
Blastomeres,Cell Nucleus,Chromosome Segregation,DNA,Female,Fertilization,Fertilization in Vitro,Humans,Male,Metaphase,Microscopy, Confocal,Microscopy, Fluorescence,Mitotic Spindle Apparatus,Oocytes,Sperm-Ovum Interactions,Spermatozoa,Tubulin,Tubulin,DNA
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA vanblerkspotcoloradoedu
Reprod. Biomed. Online
1472-6483
1202
True
15149570
