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Development of human embryos to the hatc ... ce of a monolayer of Vero cells
Development of human embryos to the hatched blastocyst stage in the presence or absence of a monolayer of Vero cells.
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In a prospective randomized study, excess embryos from 100 women undergoing in-vitro fertilization were cultured from the 2-cell to the hatched-blastocyst stage in the presence or absence of a confluent monolayer of Vero cells. The frequencies of fragmentation, developmental arrest, multinucleation and blastocyst formation were observed for 254 embryos over 7 days in culture. The number of nucleated cells, and fine structure of trophectoderm and inner cell mass were analysed at the expanded blastocyst stage on day 5.5 post-insemination. The frequency of hatching from the zona pellucida was determined between days 6 and 7 post-insemination. With respect to these developmental parameters, the findings indicate that no overt or statistically significant improvement in early human embryogenesis occurs in the co-culture system.
Van Blerkom J
Human reproduction (Oxford, England)
1993-09-01 00:00
8
9
1525-39
Adult,Animals,Blastocyst,Cell Nucleus,Culture Techniques,Embryonic and Fetal Development,Fertilization in Vitro,Humans,Prospective Studies,Vero Cells
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309
Hum. Reprod.
0268-1161
1182
True
8253948
