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Nuclear genes for mitochondrial proteins ... V of yeast cytochrome c oxidase
Nuclear genes for mitochondrial proteins. Identification and isolation of a structural gene for subunit V of yeast cytochrome c oxidase.
36
The gene for yeast cytochrome c oxidase subunit V, COX5, has been isolated from a Saccharomyces cerevisiae DNA library by complementation of a cytochrome c oxidase subunit V mutant, JM28. One complementing plasmid, YEp13-511, with a DNA insert of 4.8 kilobase pairs, has been characterized in detail. This plasmid restores respiratory competency in JM28, results in increased cytochrome c oxidase activity and a new form of subunit V in JM28 mitochondria, and is capable of selecting mRNA for subunit V. These results indicate that YEp13-511 carries the COX5 gene and that the subunit V encoded by this plasmid gene is capable of entering the mitochondrion and assembling into a functional holocytochrome c oxidase.
Cumsky MG, McEwen JE, Ko C, Poyton RO
The Journal of biological chemistry
1983-11-25 00:00
258
22
13418-21
Base Sequence,DNA Restriction Enzymes,DNA, Fungal,Electron Transport Complex IV,Genes,Macromolecular Substances,Mitochondria,Mutation,Plasmids,Protein Biosynthesis,Saccharomyces cerevisiae,DNA, Fungal,Macromolecular Substances,Electron Transport Complex IV,DNA Restriction Enzymes
J. Biol. Chem.
NIGMS GM29838, NIGMS GM30228
0021-9258
1051
True
6315696
