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Isolation and characterization of a yeas ... mitochondrial promoter for COX2
Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2.
36
Subunit II of cytochrome c oxidase is a mitochondrially encoded protein required for cellular respiration. A respiration-deficient yeast strain has been isolated and shown genetically to carry a mutation in the subunit II structural gene COX2. The respiration-deficient strain produces no subunit II polypeptide and is missing two major transcripts of the subunit II gene. The mutation, first mapped by rho- recombinational rescue to the 5' end of the gene, has been localized precisely, by DNA sequencing, 58 nucleotides upstream of the COX2 ATG initiation codon. The mutant strain carries a single nucleotide change (A to T) relative to the wild type sequence. This mutation occurs in a sequence with substantial homology to a previously identified yeast mitochondrial promoter consensus sequence. These results provide the first in vivo evidence for the importance of the consensus sequence for promoter function.
Cameron VL, Fox TD, Poyton RO
The Journal of biological chemistry
1989-08-15 00:00
264
23
13391-4
Base Sequence,DNA, Mitochondrial,Electron Transport Complex IV,Genes,Genes, Fungal,Genotype,Macromolecular Substances,Molecular Sequence Data,Mutation,Promoter Regions (Genetics),Saccharomyces cerevisiae,Species Specificity,DNA, Mitochondrial,Macromolecular Substances,Electron Transport Complex IV
Department of Biology, Ithaca College, New York 14850
J. Biol. Chem.
NIGMS GM29362, NIGMS GM30228
0021-9258
1041
True
2547760
