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A role for membrane potential in the bio ... I, a mitochondrial gene product
A role for membrane potential in the biogenesis of cytochrome c oxidase subunit II, a mitochondrial gene product.
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Subunit II of yeast cytochrome c oxidase is synthesized on mitochondrial ribosomes as a precursor containing a transient NH2-terminal presequence and is inserted into the mitochondrial inner membrane from the matrix side. Using an optimized in vitro mitochondrial translation system (McKee, E.E., and Poyton, R. O. (1984) J. Biol. Chem. 259, 9320-9331), we have examined the requirement for an electrochemical potential (delta mu H+) across the inner mitochondrial membrane during subunit II biogenesis. When mitochondrial gene products are synthesized under conditions that prevent formation of a normal delta mu H+, accumulation of unprocessed subunit II (pre-II) occurs. The majority of pre-II generated in this way is inserted into the lipid bilayer, as judged by resistance to extraction with 0.1 M Na2CO3. Therefore, it appears that a delta mu H+ is required for the normal biogenesis of subunit II, and that the delta mu H+ is required for a function other than the insertion of pre-II into the lipid bilayer of the inner membrane.
Clarkson GH, Poyton RO
The Journal of biological chemistry
1989-06-15 00:00
264
17
10114-8
DNA, Mitochondrial,Electron Transport Complex IV,Genes,Intracellular Membranes,Macromolecular Substances,Membrane Potentials,Mitochondria,Protein Biosynthesis,Saccharomyces cerevisiae,DNA, Mitochondrial,Macromolecular Substances,Electron Transport Complex IV
Department of Molecular, Cellular, and Development Biology, University of Colorado, Boulder 80309-0347
J. Biol. Chem.
NIGMS GM 30228
0021-9258
1014
True
2542322
