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Expanding the known diversity and enviro ... ylogenetic division of bacteria
Expanding the known diversity and environmental distribution of an uncultured phylogenetic division of bacteria.
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Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level candidate phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated sequences were cloned from 7 of 12 environments investigated: mainly from anaerobic sediment environments. The number of sequences representing the WS6 candidate division was increased from 3 to 60 in this study. The extent of phylogenetic divergence (sequence difference) in this candidate division was found to be among the largest of any known bacterial division. This indicates that organisms representing the WS6 phylogenetic division offer a broad diversity of undiscovered biochemical and metabolic novelty. These results provide a framework for the further study of these evidently important kinds of organisms and tools, the sequences, with which to do so.
Dojka MA, Harris JK, Pace NR
Applied and environmental microbiology
2000-04-01 00:00
66
4
1617-21
Bacteria,Cloning, Molecular,DNA, Ribosomal,Environmental Microbiology,Geologic Sediments,Humans,Molecular Sequence Data,Phylogeny,Polymerase Chain Reaction,RNA, Bacterial,RNA, Ribosomal,Variation (Genetics),DNA, Ribosomal,RNA, Bacterial,RNA, Ribosomal
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
Appl. Environ. Microbiol.
NIGMS GM34527
0099-2240
924
True
10742250
