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Comparative photocross-linking analysis ... Bacillus subtilis RNase P RNAs
Comparative photocross-linking analysis of the tertiary structures of Escherichia coli and Bacillus subtilis RNase P RNAs.
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Bacterial ribonuclease P contains a catalytic RNA subunit that cleaves precursor sequences from the 5' ends of pre-tRNAs. The RNase P RNAs from Bacillus subtilis and Escherichia coli each contain several unique secondary structural elements not present in the other. To understand better how these phylogenetically variable elements affect the global architecture of the ribozyme, photoaffinity cross-linking studies were carried out. Photolysis of photoagents attached at homologous sites in the two RNAs results in nearly identical cross-linking patterns, consistent with the homology of the RNAs and indicating that these RNAs contain a common, core tertiary structure. Distance constraints were used to derive tertiary structure models using a molecular mechanics-based modeling protocol. The resulting superimposition of large sets of equivalent models provides a low resolution (5-10 A) structure for each RNA. Comparison of these structure models shows that the conserved core helices occupy similar positions in space. Variably present helical elements that may play a role in global structural stability are found at the periphery of the core structure. The P5.1 and P15.1 helical elements, unique to the B.subtilis RNase P RNA, and the P6/16/17 helices, unique to the E.coli RNA, occupy similar positions in the structure models and, therefore, may have analogous structural function.
Chen JL, Nolan JM, Harris ME, Pace NR
The EMBO journal
1998-03-02 00:00
17
5
1515-25
Azides,Bacillus subtilis,Base Sequence,Computer Simulation,Cross-Linking Reagents,Endoribonucleases,Escherichia coli,Escherichia coli Proteins,Models, Molecular,Molecular Sequence Data,Nucleic Acid Conformation,Photolysis,RNA, Bacterial,RNA, Catalytic,Ribonuclease P,Azides,Cross-Linking Reagents,Escherichia coli Proteins,RNA, Bacterial,RNA, Catalytic,4-azidophenacyl bromide,Endoribonucleases,Ribonuclease P,ribonuclease P, E coli
Departments Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720-3102, USA
EMBO J.
NIGMS GM34527
0261-4189
10.1093/emboj/17.5.1515
907
True
9482748
