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Community and cultivation analysis of arsenite oxidizing biofilms at Hot Creek
Community and cultivation analysis of arsenite oxidizing biofilms at Hot Creek.
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At Hot Creek in California, geothermally derived arsenite is rapidly oxidized to arsenate. This process is mediated by microorganisms colonizing the surfaces of submerged aquatic macrophytes in the creek. Here we describe a multifaceted approach to characterizing this biofilm community and its activity. Molecular techniques were used to describe the community as a function of 16S-rRNA gene diversity. Cultivation-based strategies were used to enumerate and isolate three novel arsenite oxidizers, strains YED1-18, YED6-4 and YED6-21. All three strains are beta-Proteobacteria, of the genus Hydrogenophaga. Because these strains were isolated from the highest (i.e. million-fold) dilutions of disrupted biofilm suspensions, they represent the most numerically significant arsenite oxidizers recovered from this community. One clone (Hot Creek Clone 44) obtained from an inventory of the 16S rDNA sequence diversity present in the biofilm was found to be 99.6% identical to the 16S rDNA sequence of the isolate YED6-21. On the basis of most probable number (MPN) analyses, arsenite-oxidizing bacteria were found to account for 6-56% of the cultivated members of the community. Using MPN values, we could estimate an upper bound on the value of V(max) for the community of 1 x 10(-9)micromole arsenite min(-1) cell(-1). This estimate represents the first normalization of arsenite oxidation rates to MPN cell densities for a microbial community in a field incubation experiment.
Salmassi TM, Walker JJ, Newman DK, Leadbetter JR, Pace NR, Hering JG
Environmental microbiology
2006-01-01 00:00
8
1
50-9
Arsenites,Base Sequence,Biofilms,California,Cluster Analysis,Hydrogenophilaceae,Kinetics,Molecular Sequence Data,Oxidation-Reduction,Phylogeny,Polymorphism, Restriction Fragment Length,RNA, Ribosomal, 16S,Sequence Analysis, DNA,Variation (Genetics),Water Microbiology,Arsenites,RNA, Ribosomal, 16S,arsenite
Environmental Science and Engineering, California Institute of Technology, Pasadena, 91125, USA tsalmascalstatelaedu
Environ. Microbiol.
1462-2912
10.1111/j.1462-2920.2005.00862.x
EMI862
905
True
16343321
