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The AP-3 adaptor complex is essential fo ... transport to the yeast vacuole
The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole.
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Three distinct adaptor protein (AP) complexes involved in protein trafficking have been identified. AP-1 and AP-2 mediate protein sorting at the trans-Golgi network and plasma membrane, respectively, whereas the function of AP-3 has not been defined. A screen for factors specifically involved in transport of alkaline phosphatase (ALP) from the Golgi to the vacuole/lysosome has identified Ap16p and Ap15p of the yeast AP-3 complex. Deletion of each of the four AP-3 subunits results in selective mislocalization of ALP and the vacuolar t-SNARE, Vam3p (but not CPS and CPY), while deletion of AP-1 and AP-2 subunits has no effect on vacuolar protein delivery. This study, therefore, provides evidence that the AP-3 complex functions in cargo-selective protein transport from the Golgi to the vacuole/lysosome.
Cowles CR, Odorizzi G, Payne GS, Emr SD
Cell
1997-10-03 00:00
91
1
109-18
Adaptor Protein Complex alpha Subunits,Adaptor Proteins, Vesicular Transport,Alkaline Phosphatase,Biological Transport,Carboxypeptidase C,Carboxypeptidases,Dipeptidyl Peptidases,Fungal Proteins,Golgi Apparatus,Lysosomes,Membrane Proteins,Monomeric Clathrin Assembly Proteins,Qa-SNARE Proteins,Recombinant Fusion Proteins,Saccharomyces cerevisiae,Saccharomyces cerevisiae Proteins,Sequence Deletion,Vacuoles,Adaptor Protein Complex alpha Subunits,Adaptor Proteins, Vesicular Transport,Fungal Proteins,Membrane Proteins,Monomeric Clathrin Assembly Proteins,PEP12 protein, S cerevisiae,Qa-SNARE Proteins,Recombinant Fusion Proteins,Saccharomyces cerevisiae Proteins,VAM3 protein, S cerevisiae,clathrin assembly protein AP180,Alkaline Phosphatase,Carboxypeptidases,Dipeptidyl Peptidases,STE13 protein, S cerevisiae,Carboxypeptidase C,carboxypeptidase S, S cerevisiae
Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California, San Diego 92093-0668, USA
Cell
NCI CA 58689, NCI CA58689, NIGMS GM32703
0092-8674
S0092-8674(01)80013-1
847
True
9335339
