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EBNA2 and activated Notch induce expression of BATF
EBNA2 and activated Notch induce expression of BATF.
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The immortalization of human B lymphocytes by Epstein-Barr virus (EBV) requires the virus-encoded transactivator EBNA2 and the products of both viral and cellular genes which serve as EBNA2 targets. In this study, we identified BATF as a cellular gene that is up-regulated dramatically within 24 h following the infection of established and primary human B cells with EBV. The transactivation of BATF is mediated by EBNA2 in a B-cell-specific manner and is duplicated in non-EBV-infected B cells by the expression of mammalian Notch proteins. In contrast to other target genes activated by EBNA2, the BATF gene encodes a member of the AP-1 family of transcription factors that functions as a negative regulator of AP-1 activity and as an antagonist of cell growth. A potential role for BATF in promoting EBV latency is supported by studies in which BATF was shown to negatively impact the expression of a BZLF1 reporter gene and to reduce the frequency of lytic replication in latently infected cells. The identification of BATF as a cellular target of EBV provides important new information on how programs of viral and cellular gene expression may be coordinated to promote viral latency and control lytic-cycle entry.
Johansen LM, Deppmann CD, Erickson KD, Coffin WF, Thornton TM, Humphrey SE, Martin JM, Taparowsky EJ
Journal of virology
2003-05-01 00:00
77
10
6029-40
B-Lymphocytes,Basic-Leucine Zipper Transcription Factors,Cell Line,Cells, Cultured,Epstein-Barr Virus Nuclear Antigens,Gene Expression Regulation, Viral,Hela Cells,Herpesvirus 4, Human,Humans,Membrane Proteins,Receptors, Notch,Trans-Activation (Genetics),Transcription Factors,Transcription, Genetic,Viral Proteins,Virus Latency,Basic-Leucine Zipper Transcription Factors,Batf protein, mouse,EBNA-2 protein, Human herpesvirus 4,Epstein-Barr Virus Nuclear Antigens,Membrane Proteins,Receptors, Notch,Transcription Factors,Viral Proteins
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA
J. Virol.
NIAID AI01537, NCI CA64610, NCI CA78264, NIGMS GM08298, NCI T32 CA09634
0022-538X
823
True
12719594
