Document Actions
Yin Yang 1 represses alpha-myosin heavy ... pathologic cardiac hypertrophy
Yin Yang 1 represses alpha-myosin heavy chain gene expression in pathologic cardiac hypertrophy.
24
In the work presented here, we elucidate a mechanism for the repression of alpha-myosin heavy chain (MyHC) during pathological cardiac hypertrophy. We demonstrate that the transcription factor Yin Yang 1 (YY1) significantly decreases endogenous alpha-MyHC mRNA and protein expression in neonatal rat ventricular myocytes. Furthermore, mutation of the YY1 binding sites in the proximal rat alpha-MyHC promoter increases promoter activity and alleviates YY1-mediated repression of the promoter. Despite the presence of 5 sites that bind YY1, only one site, located at -94bp of the rat alpha-MyHC promoter, is both necessary and sufficient for pathological repression of the promoter by phorbol esters, revealing a unique mechanism for the repression of alpha-MyHC expression during cardiac hypertrophy.
Mariner PD, Luckey SW, Long CS, Sucharov CC, Leinwand LA
Biochemical and biophysical research communications
2005-01-07 00:00
326
1
79-86
Animals,Animals, Newborn,Cardiomegaly,Cells, Cultured,DNA-Binding Proteins,Erythroid-Specific DNA-Binding Factors,Gene Expression Regulation,Heart Ventricles,Myocytes, Cardiac,Myosin Heavy Chains,Rats,Recombinant Proteins,Structure-Activity Relationship,Transcription Factors,Ventricular Myosins,YY1 Transcription Factor,DNA-Binding Proteins,Erythroid-Specific DNA-Binding Factors,Myosin Heavy Chains,Recombinant Proteins,Transcription Factors,YY1 Transcription Factor,Yy1 protein, rat,Ventricular Myosins
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
Biochem. Biophys. Res. Commun.
0006-291X
10.1016/j.bbrc.2004.11.008
S0006-291X(04)02558-6
799
True
15567155
