Document Actions
Aryl hydrocarbon induction of rat cytoch ... reased precursor RNA processing
Aryl hydrocarbon induction of rat cytochrome P-450d results from increased precursor RNA processing
22
We have previously demonstrated that cytochrome P-450d mRNA accumulation is induced at a posttranscriptional level by 3-methylcholanthrene (MCA) in primary cultures of rat hepatocytes grown in serum-free hormonally defined medium. Using dactinomycin chase experiments in this culture system, we found that MCA had no effect on the P-450d mRNA half-life. In addition, induction of P-450d occurred both in the presence and in the absence of protein synthesis inhibitors. An analysis of nuclear precursors showed that the accumulation of the primary transcript of the P-450d gene was induced to the same extent as that of the mature mRNA after MCA treatment and that the pattern of accumulation of precursors differed between treated and control liver cells. Since P-450d induction is thought to be a receptor-mediated event, these data are consistent with a model in which a direct interaction occurs between the receptor-ligand complex and the primary transcript.
Silver G, Krauter KS
Molecular and cellular biology
1990-12-01 00:00
10
12
6765-8
Animals,Cell Nucleus,Cells, Cultured,Cycloheximide,Cytochrome P-450 Enzyme System,Enzyme Induction,Kinetics,Liver,Methylcholanthrene,RNA Precursors,RNA Processing, Post-Transcriptional,RNA, Messenger,Rats,RNA Precursors,RNA, Messenger,Methylcholanthrene,Cycloheximide,Cytochrome P-450 Enzyme System
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Mol. Cell. Biol.
NIADDK AM17702, NCI CA39553, NCI P30-CA13330
0270-7306
0
False
2247082
