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The trkB tyrosine protein kinase is a re ... ophic factor and neurotrophin-3
The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3.
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trkB is a tyrosine protein kinase gene highly related to trk, a proto-oncogene that encodes a receptor for nerve growth factor (NGF) and neurotrophin-3 (NT-3). trkB expression is confined to structures of the central and peripheral nervous systems, suggesting it also encodes a receptor for neurotrophic factors. Here we show that brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of gp145trkB, one of the receptors encoded by trkB. BDNF and NT-3 can induce DNA synthesis in quiescent NIH 3T3 cells that express gp145trkB. Cotransfection of plasmids encoding gp145trkB and BDNF or NT-3 leads to transformation of recipient NIH 3T3 cells. In these assays, BDNF elicits a response at least two orders of magnitude higher than NT-3. Finally, 125I-NT-3 binds to NIH 3T3 cells expressing gp145trkB; binding can be competed by NT-3 and BDNF but not by NGF. These findings indicate that gp145trkB may function as a neurotrophic receptor for BDNF and NT-3.
Klein R, Nanduri V, Jing SA, Lamballe F, Tapley P, Bryant S, Cordon-Cardo C, Jones KR, Reichardt LF, Barbacid M
Cell
1991-07-26 00:00
66
2
395-403
Animals,Brain,Brain-Derived Neurotrophic Factor,Cell Division,Cell Line,Kinetics,Membrane Glycoproteins,Mice,Nerve Growth Factors,Nerve Tissue Proteins,Neurotrophin 3,Plasmids,Protein-Tyrosine Kinases,Receptor, trkB,Receptors, Cell Surface,Receptors, Nerve Growth Factor,Transfection,Brain-Derived Neurotrophic Factor,Membrane Glycoproteins,Nerve Growth Factors,Nerve Tissue Proteins,Neurotrophin 3,Receptors, Cell Surface,Receptors, Nerve Growth Factor,Ntrk2 protein, mouse,Protein-Tyrosine Kinases,Receptor, trkB
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000
Cell
0092-8674
605
True
1649702
