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sur-2, a novel gene, functions late in t ... bditis elegans vulval induction
sur-2, a novel gene, functions late in the let-60 ras-mediated signaling pathway during Caenorhabditis elegans vulval induction.
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We describe here a new gene acting downstream of let-60 ras in the vulval signaling pathway of Caenorhabditis elegans. The sur-2 (suppressor of ras) gene is defined by eight mutations identified in a genetic screen for suppressors of the Multivulva phenotype of let-60(n1046), an activated let-60 ras mutation. sur-2 mutations result in pleiotropic, incompletely penetrant phenotypes that include a Vulvaless phenotype in hermaphrodites, defects in development of the male tail, gonadal abnormalities, and larval lethality, indicating a role for the sur-2 gene product in multiple developmental events. Genetic epistasis analyses suggest that sur-2 is required late in the vulval signaling pathway, downstream of let-60 Ras, and is likely to act downstream of the Raf/MAP Kinase cascade. We cloned the sur-2 gene by DNA-mediated transformation and have shown that it encodes a novel protein. We also show that a sur-2::lacZ transgene is expressed in the vulval precursor cells at the time of vulval determination.
Singh N, Han M
Genes & development
1995-09-15 00:00
9
18
2251-65
Amino Acid Sequence,Animals,Base Sequence,Caenorhabditis elegans,Caenorhabditis elegans Proteins,Embryonic Induction,Exons,Female,Gene Expression Regulation, Developmental,Genes, Helminth,Helminth Proteins,Introns,Models, Biological,Molecular Sequence Data,Mutation,Restriction Mapping,Signal Transduction,Suppression, Genetic,Transcription Factors,Vulva,ras Proteins,Caenorhabditis elegans Proteins,Helminth Proteins,Sur-2 protein, C elegans,Transcription Factors,let-60 protein, C elegans,ras Proteins
Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder 80309-0347, USA
Genes Dev.
NIGMS R01 GM 47869
0890-9369
537
True
7557379
