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The leucine-rich repeat protein SUR-8 en ... orms a complex with Ras and Raf
The leucine-rich repeat protein SUR-8 enhances MAP kinase activation and forms a complex with Ras and Raf.
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Caenorhabditis elegans sur-8 encodes a positive regulator of Ras signaling. We investigated the mechanism by which the human Sur-8 homolog can positively regulate Ras-MAP kinase signaling in mammalian cells. Sur-8 expression enhances Ras- or EGF-induced Raf and ERK activation but has no effect on ERK activation induced by active Raf or MEK. Furthermore, Sur-8 expression does not increase AKT or JNK activation. Sur-8 interacts with Ras and Raf and is able to form a ternary complex with the two proteins. Thus, Sur-8 may function as a scaffold that enhances Ras-MAP kinase signal transduction by facilitating the interaction between Ras and Raf.
Li W, Han M, Guan KL
Genes & development
2000-04-15 00:00
14
8
895-900
Adaptor Proteins, Signal Transducing,Blotting, Western,Caenorhabditis elegans Proteins,Carrier Proteins,Cell Line,DNA, Complementary,Dose-Response Relationship, Drug,Enzyme Activation,Epidermal Growth Factor,Glutathione Transferase,Humans,MAP Kinase Signaling System,Plasmids,Precipitin Tests,Protein Binding,Protein-Serine-Threonine Kinases,Proto-Oncogene Proteins,Proto-Oncogene Proteins c-akt,Proto-Oncogene Proteins c-raf,Signal Transduction,Transfection,Two-Hybrid System Techniques,Up-Regulation,ras Proteins,Adaptor Proteins, Signal Transducing,Caenorhabditis elegans Proteins,Carrier Proteins,DNA, Complementary,Proto-Oncogene Proteins,SUR-8 protein, C elegans,Epidermal Growth Factor,Glutathione Transferase,AKT1 protein, human,Protein-Serine-Threonine Kinases,Proto-Oncogene Proteins c-akt,Proto-Oncogene Proteins c-raf,ras Proteins
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA
Genes Dev.
0890-9369
528
True
10783161
