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The developmental timing regulator AIN-1 ... toplasmic P bodies in C elegans
The developmental timing regulator AIN-1 interacts with miRISCs and may target the argonaute protein ALG-1 to cytoplasmic P bodies in C. elegans.
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In metazoans, microRNAs (miRNAs) carry out various regulatory functions through association with multiprotein miRNA-induced silencing complexes (miRISCs) that contain Dicer and Argonaute proteins. How miRNAs regulate the expression of their mRNA targets remains a major research question. We have identified the C. elegans ain-1 gene through a genetic suppressor screen and shown that it functions with the heterochronic genetic pathway that regulates developmental timing. Biochemical analysis indicates that AIN-1 interacts with protein complexes containing an Argonaute protein, Dicer, and miRNAs. AIN-1 shares homology with the candidate human neurological disease protein GW182, shown to localize in cytoplasmic processing bodies that are sites of mRNA degradation and storage. A functional AIN-1::GFP also localizes at the likely worm processing bodies. When coexpressed from transgenes, AIN-1 targets ALG-1 to the foci. These results suggest a model where AIN-1 regulates a subset of miRISCs by localization to the processing bodies, facilitating degradation or translational inhibition of mRNA targets.
Ding L, Spencer A, Morita K, Han M
Molecular cell
2005-08-19 00:00
19
4
437-47
Animals,Caenorhabditis elegans,Caenorhabditis elegans Proteins,Carrier Proteins,Cell Differentiation,Cytoplasmic Structures,Endoribonucleases,Gene Expression Regulation, Developmental,Gene Silencing,MicroRNAs,Molecular Sequence Data,AIN-1 protein, C elegans,Caenorhabditis elegans Proteins,Carrier Proteins,MicroRNAs,Endoribonucleases,dcr-1 protein, C elegans
Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder, 80309, USA
Mol. Cell
1097-2765
10.1016/j.molcel.2005.07.013
S1097-2765(05)01477-2
525
True
16109369
