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The Caenorhabditis elegans ect-2 RhoGEF ... migration of epidermal P cells
The Caenorhabditis elegans ect-2 RhoGEF gene regulates cytokinesis and migration of epidermal P cells.
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A reduction-of-function mutation in ect-2 was isolated as a suppressor of the Multivulva phenotype of a lin-31 mutation. Analysis using markers indicates that this mutation causes defects in both the cytokinesis and migration of epidermal P cells, phenotypes similar to those caused by expressing a rho-1 dominant-negative construct. ect-2 encodes the Caenorhabditis elegans orthologue of the mouse Ect2 and Drosophila Pebble that function as guanine nucleotide exchange factors (GEFs) for Rho GTPases. The ect-2Colon, two colonsGFP reporter is expressed in embryonic cells and P cells. RNA interference of ect-2 causes sterility and embryonic lethality, in addition to the P-cell defects. We have determined the lesions of two ect-2 alleles, and described their differences in phenotypes in specific tissues. We propose a model in which ECT-2GEF not only activates RHO-1 for P-cell cytokinesis, but also collaborates with UNC-73GEF and at least two Rac proteins to regulate P-cell migration.
Morita K, Hirono K, Han M
EMBO reports
2005-12-01 00:00
6
12
1163-8
Animals,Base Sequence,Caenorhabditis elegans,Caenorhabditis elegans Proteins,Cell Movement,Chromosome Mapping,Chromosomes,Consensus Sequence,Cytokinesis,Epithelial Cells,Female,Genes, Helminth,Genes, Reporter,Genetic Markers,Green Fluorescent Proteins,Guanine Nucleotide Exchange Factors,Models, Biological,Molecular Sequence Data,Promoter Regions (Genetics),Protein Structure, Tertiary,Sequence Deletion,Caenorhabditis elegans Proteins,Ect-2 protein, C elegans,Genetic Markers,Guanine Nucleotide Exchange Factors,rho guanine nucleotide exchange factor p115,Green Fluorescent Proteins
Department of MCD Biology, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA
EMBO Rep.
NIGMS GM47869
1469-221X
10.1038/sj.embor.7400533
7400533
522
True
16170304
